Journal: Blood Advances

Article Title: TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia

doi: 10.1182/bloodadvances.2022008405

Figure Lengend Snippet: Gal-9 ligation to TIM-3 induces LRP6 signalosome formation and subsequent β-catenin accumulation in AML. (A) WB analysis of β-catenin accumulation induced by stimulation with Gal-9 in the presence of inhibitors in KASUMI-3 cells. KASUMI-3 cells were stimulated with Gal-9 in the presence of dimethyl sulfoxide, 10 μM of U0126 (MEK1/2 inhibitor), 10 μM of LY294002 (PI3K inhibitor), or 200 ng/mL of DKK-1, and their total cell lysates subjected to WB analysis. (B) Summarized data of Gal-9 stimulation–induced changes in the accumulation of β-catenin from 3 independent experiments. (C) Extent of β-catenin translocation to the nucleus evaluated using the ArrayScan system compared with that observed in nonstimulated controls. Data in panels B and C are presented as mean ± SEM, ∗ P < .05. (D) Immunoblotting analysis of total lysates (left) and IP lysates (right) of Gal-9–stimulated primary TIM-3 + AML samples. Cells were lysed and subjected to IP with an anti-LRP6 antibody or normal mouse IgG. A representative result of TIM-3 + primary AML cells out of 3 independent cases (AML#4, #9, and #13) is shown. (E) WB analysis of Gal-9 stimulation–induced phosphorylation of LRP6 at Thr1479 in mock-transfected and TIM-3-OX THP-1 cells. (F) WB analysis of cell lysates from TIM-3 + AML cells (left) and TIM-3 − AML cells (right). TIM-3 + AML cells were stimulated with Gal-9 in the absence or presence of DKK-1 (200 ng/mL). Representative results out of 4 independent TIM-3 + AML cells (AML#1, #2, #4, and #16) and 2 TIM-3 − AML cells (AML#10 and #11) are shown. n.s., no significant difference.

Article Snippet: For the assays with inhibitors, cells were incubated in the presence of A-419259 (Cayman Chemical), U0126 (Promega), LY294002 (Abcam), human dickkopf Wnt signaling pathway inhibitor 1 (DKK-1) recombinant protein (Peprotech), and a Src family inhibitors set including protein phosphatase 1 (PP1), PP2, and aminogenistein (AMGT) (proteinkinase.de) for 2 hours before stimulation with Gal-9.

Techniques: Ligation, Translocation Assay, Western Blot, Transfection

Journal: Geburtshilfe und Frauenheilkunde

Article Title: Comparison of Maternal Serum Levels and Placental mRNA Levels of Dickkopf-1 in Preeclamptic and Normal Pregnant Women at Delivery

doi: 10.1055/a-1557-1234

Figure Lengend Snippet: Correlation between maternal DKK1 serum levels and existence with symptoms in PE and NP women. a PE women with diagnosis of one or more symptoms had decreased maternal serum DKK1 levels compared with PE cases with no symptom. b NP women showed no differences in maternal serum DKK1 levels between cases diagnosed with edema and cases without edema. Results are presented as Mean (Range). p-value is tested using the non-parametric Mann-Whitney-U-test. n: number of cases.

Article Snippet: Samples were tested using TaqMan Gene Expression Assays by Life Technologies (Dickkopf WNT signaling pathway inhibitor 1 [DKK1], [catalog no. Hs00183740_m1], Catenin beta1 [CTNNB1] [catalog no. Hs00355045_m1], and Actin beta [ACTB] [caltalog no. Hs03929097_m1]) and Applied Biosystems 7500 Fast Real-Time PCR System.

Techniques: MANN-WHITNEY

Journal: Geburtshilfe und Frauenheilkunde

Article Title: Comparison of Maternal Serum Levels and Placental mRNA Levels of Dickkopf-1 in Preeclamptic and Normal Pregnant Women at Delivery

doi: 10.1055/a-1557-1234

Figure Lengend Snippet: Table 2 Correlation of maternal serum DKK1 levels with preeclampsia features.

Article Snippet: Samples were tested using TaqMan Gene Expression Assays by Life Technologies (Dickkopf WNT signaling pathway inhibitor 1 [DKK1], [catalog no. Hs00183740_m1], Catenin beta1 [CTNNB1] [catalog no. Hs00355045_m1], and Actin beta [ACTB] [caltalog no. Hs03929097_m1]) and Applied Biosystems 7500 Fast Real-Time PCR System.

Techniques:

Journal: Geburtshilfe und Frauenheilkunde

Article Title: Comparison of Maternal Serum Levels and Placental mRNA Levels of Dickkopf-1 in Preeclamptic and Normal Pregnant Women at Delivery

doi: 10.1055/a-1557-1234

Figure Lengend Snippet: Placental gene expression levels of DKK1 and β-catenin in NP and PE cases. a PE cases had significantly lower placental DKK1 mRNA levels compared with NP cases. b Placental β-catenin mRNA levels in PE cases were also lower than those in NP cases but the difference was not statistically significant. Results are presented as Mean (Range). p-value is tested using the non-parametric Mann-Whitney-U-test. n: number of cases.

Article Snippet: Samples were tested using TaqMan Gene Expression Assays by Life Technologies (Dickkopf WNT signaling pathway inhibitor 1 [DKK1], [catalog no. Hs00183740_m1], Catenin beta1 [CTNNB1] [catalog no. Hs00355045_m1], and Actin beta [ACTB] [caltalog no. Hs03929097_m1]) and Applied Biosystems 7500 Fast Real-Time PCR System.

Techniques: Expressing, MANN-WHITNEY